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- W2369718423 abstract "A novel biosensor for the determination of H2O2 was developed based on the immobilization of horseradish peroxidase(HRP)-labeled gold nanoparticles in nano-Al2O3-chitosan biocomposite matrix.The large surface area of Al2O3 nanoparticles and the biocompatibility and physiological inertness of chitosan resulted that the enzyme not only had a high loading,but also retained better bioactivity.The electrochemistric characterize of the film was investigated by cyclic voltammetry.In the optimized conditions,the biosensor exhibited a wide linear response to H2O2 concentration ranging from 0.3 to 270 μmol/L with a limit of detection(S/N=3) down to 0.1 μmol/L.Except for ascorbic acid,other biologic compounds probably existed in the sample,such as glucose,and various amino acids,as well as biologic relevant cations and anions,did not disturb the determination of H2O2.The apparent Michaelis-Menten constants of the enzyme electrode were calculated to be 0.13 mmol/L for H2O2.The designed sensor showed good repeatability and stability with spiked recoveries of 98%and RSD of 3.8%.The method was rapid,simple and cheap,and showed propromise for applying in the development of other enzyme-based biosensors." @default.
- W2369718423 created "2016-06-24" @default.
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- W2369718423 date "2010-01-01" @default.
- W2369718423 modified "2023-09-26" @default.
- W2369718423 title "A Novel HRP Biosensor Based on Al_2O_3-Chitosan Nanocomposite and Au Nanoparticle" @default.
- W2369718423 hasPublicationYear "2010" @default.
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