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- W2369730369 abstract "Objectives To develop a prophylactic and therapeutic vaccine against HPV16 with the E7C subgene possessing the antigenicity of E7 and no transformational activity, and trying to explore more active vaccine for inducing cellular immunity with B7 1. Methods In these experiments, the E7C was amplified by PCR and then inserted into eukaryotic expression plasmid for constructing pLNCE7C. Being confirmed the ability to express in eukaryotic cells in vitro, the pLNCE7C plasmid alone or associated with mouse B7 1 was inoculated directly into the muscles on the posterior leg of C57BL/6 mice , the activity of cytotoxic T lymphocytes (CTL) isolated from the immunized mice were analyzed in vitro with 51 Cr specific release assay. Results The CTLs from immunized mice were activated efficaciously by naked E7 expression plasmid ; higher activity of CTL could be produced by B7 1 synergizing with E7C through immunizing with the mixture of both expressing plasmids. Conclusions E7C subgene is proved suitable for developing DNA vaccine, and the B7 1 is worthy for exploiting widely." @default.
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- W2369730369 date "1999-01-01" @default.
- W2369730369 modified "2023-09-25" @default.
- W2369730369 title "Human papillomavirus 16 E7-specific CTL induction through mouse B7-1 costimulating with E7C subgene" @default.
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