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- W2369776671 abstract "In order to probe into the method of the more rapid,simple and effective extraction of nuclic acids from minimal tissue ,the nuclic acids of 25 samples from gastric mucosa were extracted by improved splitting method.We compared it with the phenol extraction method and the digesting boiling method (25 cases each group).The obtained nuclic acids were detected by 1% agarose electrophoresis and were quantified by ultraviolet spectrophotometrin method.The results showed that the nuclic acids'purity extracted by the improved splitting method was similar with the results of the phenol extraction method and their quantity was more,the required time was the shortest.The improved splitting method can not only be used to extract DNA but aslo to RNA,owing to including Rnase inhibitor in the splitting solution,so the obtained RNA by the method may be used to RT PCR.Conclusion:the improved splitting method is an effective and practical method for the extraction of DNA and RNA from minimal tissues." @default.
- W2369776671 created "2016-06-24" @default.
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- W2369776671 date "1999-01-01" @default.
- W2369776671 modified "2023-09-25" @default.
- W2369776671 title "Study on the Extraction of Nrclic Acids from Minimal Tissue by Improved Splitting Method" @default.
- W2369776671 hasPublicationYear "1999" @default.
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