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- W2369862016 abstract "Objective To explore whether bone marrow non-adherent mesenchymal stem cells(BM-NA-MSC) exist in mouse bone marrow;whether the NA-MSC can form fibroblastic colony units forming(CFU-F) and whether they have the ability of differentiating into adipocyte,chondrocyte and osteoblast cells,which are typicals of BM-NA-MSC.Methods Bone marrow cells of mouse were isolated and cultured in vitro,non-adherent-bone marrow cells(NA-BMC) were repeatedly-transterred into new dishes every day for 5 times,every dish was stained with methylene blue after 12 days cell culture for CFU-F.For the detection of differentiation,NA-BMC were collected at the 4th day of the culture,3 conditioned medium were used to induce the NA-BMC into adipocyte,chondrocyte and osteoblast cells.Oil red staining,immunohistochemical staining of type Ⅱ collagen and alizarin red staining were developed respectively to detect the differentiation of NA-BMC.Results In mice,not only total bone marrow cell could give rise to CFU-F,but also NA-BMC could give rise to CFU-F continuously.They could differentiate into adipocyte which appeared oil-red positive,chondrocyte expressing typeⅡ collagen and osteoblast which could calcify after induced with conditioned medium in vitro.Conclusions It indicate that there are NA-MSC in mouse bone marrow.They can give rise to CFU-F continuously and differentiate into adipocyte,chondrocyte osteoblast,which appears some pluripotency and provides a promising for being used into cell therapetics." @default.
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- W2369862016 date "2008-01-01" @default.
- W2369862016 modified "2023-09-23" @default.
- W2369862016 title "Isolation in Vitro and Identification of Non-Adherent Bone Marrow Mesenchymal Stem Cells of Mouse" @default.
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