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- W2369901118 abstract "Objective To investigate the optimal ultrasonic irradiation conditions of PC3 and LNcap cells,and to compare the effect of ultrasound irradiation on the cell membrane permeability and cell survival rate between the two types of human prostate cancer cells.Methods PC3 and LNcap cells were exposed to 1 MHz,continuous waves in 37℃ water bath,respectively.Different parameters such as ultrasound intensity,exposure duration and microbubble concentration were applied.The cell luminosity factor was detected by flow cytometry,and the cell survival rate was calculated with trypan blue exclusion.Results The optimal parameters for both types of cells were intensity 0.9 W/cm2,exposure duration 40 s,contrast agent concentration 20%.When exposed to ultrasound intensity of 1.5 W/cm2 for 20 s in the presence of 20% contrast agent,the luminosity factor of PC3 cells was higher than that of LNcap cells.The luminosity factor of PC3 cells was lower than or the same as that of LNcap cells when exposed to other parameters.On conditions of different parameters,the survival rate of PC3 cells was always higher than that of LNcap cells(P0.05).Conclusion Optimizing the exposure parameters can safely and efficiently increase the cell membrane permeability." @default.
- W2369901118 created "2016-06-24" @default.
- W2369901118 creator A5060071396 @default.
- W2369901118 date "2011-01-01" @default.
- W2369901118 modified "2023-09-27" @default.
- W2369901118 title "Uptake of FD500 by human prostate cancer cells mediated by ultrasound and microbubble contrast agent" @default.
- W2369901118 hasPublicationYear "2011" @default.
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