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- W2369919893 abstract "Objective To study epidemic characteristics and trends of Vibrio cholerae(V.cholerae) serotype Ogawa in Guangzhou area and analyze the pathogenicity-related genes types and pulse field gel electrophoresis(PFGE)types of V.cholerae serotype Ogawa strains isolated from 2000 to 2005. Methods Phage-biotyping and multiplex polymerase chain reaction assay were applied in detecting the four pathogenicity-related genes of Ogawa V.cholerae.PFGE was employed for the molecular subtyping and PFGE results were then analyzed with BioNumerics Version 4.0 software.Results There were three pathogenicity-related gene types found in isolated V.cholerae Ogawa strains,which were named as type A,B and C.Ninety-eight percent of the isolates from people infected belonged to pathogenicity-related genes type A and forty-five percent of environmental strains belonged to type C.Thirty-nine distinct PFGE types were obtained from all 82 isolated strains.The same PFGE subtyping characteristics existed in both strains isolated from outbreak and those from sporadic events at the same year,while different PFGE subtypes were found in the environmental strains.The local strains and those imported from other provinces and even from foreign countries showed the similar subtyping sometimes.Conclusions There is a correlative relationship among the results of phage- biotyping,multiplex polymerase chain reaction assay and PFGE.Molecular subtyping database of V.cholerae should be established in order to prevent and control outbreak of cholera." @default.
- W2369919893 created "2016-06-24" @default.
- W2369919893 creator A5008558040 @default.
- W2369919893 date "2007-01-01" @default.
- W2369919893 modified "2023-09-24" @default.
- W2369919893 title "Molecular subtyping of Vibrio cholerae serotype Ogawa isolated in Guangzhou area from 2000 to 2005" @default.
- W2369919893 hasPublicationYear "2007" @default.
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