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- W2369933536 abstract "Objective To isolate rat ventricular myocytes,record single channel Ca2+ current and analyze its electrophysiological characteristics.Methods Rat ventricular myocytes were isolated with collagenase-digestion method;L-type calcium channel currents were recorded with the cell-attached and inside-out configurations of patch clamp technology,and analyzed with pClamp10 software.Results The survived rat ventricular myocytes separated in current study were rod-shaped or cylindrical with clear stripes.The currents of L-type calcium channel were recorded and analyzed:the average current amplitude was(1.13 ± 0.01)pA,conductance was 19 pS,the average open time constant was(1.35 ± 0.03)ms,and the average closing time constant was(46.75 ± 10.04)ms.In addition,the run-down phenomenon of the L-type calcium channel was observed in the inside-out mode.Conclusion The separated rat ventricular myocytes were suitable for single channel recording of patch clamp technique;the basic characteristics of L-type Ca2+ channel were consistent with the literature." @default.
- W2369933536 created "2016-06-24" @default.
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- W2369933536 date "2013-01-01" @default.
- W2369933536 modified "2023-09-26" @default.
- W2369933536 title "Recording of Single Channel Ca~(2+) Current and the Characterization of Its Electrophysiological Characteristics in Rat Ventricular Myocytes" @default.
- W2369933536 hasPublicationYear "2013" @default.
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