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- W2369940959 abstract "Objective To construct tissue-engineered skin with skin appendages by inserting isolated hair follicle units and to explore the effect of hair follicle units on the construction of tissue-engineered skin.Methods Single undamaged hair follicle units were dissected by surgical blades.Then hair follicle units were inserted to the engineered dermis including Rat tail tendon collagen type I,fibroblasts and Keratinocytes.After 1 week of submerged culture and 3 weeks of air-liquid culture,tissue-engineered skin and the hair follicle were detected by inverted microscope,HE staining and immunohistochemical staining.Results Hair follicle morphology could maintain more than 21 days without hair papilla damage,which was longer than those of free-floating culture.After 4 weeks,skin appendages such as hair follicle and sebaceous glands were found in the tissue engineered skin by HE staining.A continuous basement membrane was observed and associated with the expression of laminin and collagen IV by immunohistochemical staining.Keratinocytes seeded on the tissue with hair follicle units formed a stratified and cornified epidermis and expressed typical markers of differentiation(keratin4,keratin10/13).Conclusion Inserting hair follicle units could promote the differentiation and mature of tissue engineered skin,which could provide a new way to construct tissue-engineered skin." @default.
- W2369940959 created "2016-06-24" @default.
- W2369940959 creator A5046150365 @default.
- W2369940959 date "2013-01-01" @default.
- W2369940959 modified "2023-09-24" @default.
- W2369940959 title "Hair Follicle Units Improving the Construction of Tissue Engineered Skin in Vitro" @default.
- W2369940959 hasPublicationYear "2013" @default.
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