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- W2369946239 abstract "Based on published AIV(H5N1) genome sequence,a fragment of about 750bp long was amplified by PCR technique with specific primers using biological software DNAStar to analysis.Then the amplified product was directionally cloning into pET30aexpression vector.After identifying with enzyme cut and sequencing,the recombinant plasmid was transformed into E.coli BL21(DE3).The recombinant protein M1was expressed in inclusion body form in E coli after induction with IPTG.After denaturation,purification and renaturation,the concentration of purified protein was 0.656 mg / mL,Western blotting showed the recombinant protein had a good immunogenicity.The purified protein immunized BALB / c mice,indirect ELISA showed that the recombinant protein could produce specific antibodies anti-M1IgG,which had a good immunogenicity.That is a better basic for diagnostic and genetically engineering vaccine research on H5N1influenza virus." @default.
- W2369946239 created "2016-06-24" @default.
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- W2369946239 date "2012-01-01" @default.
- W2369946239 modified "2023-09-26" @default.
- W2369946239 title "Immunogenicity Identification of M1Matrix Protein of A/duck/Fujian/31/2007 H5N1Subtype Influenza Virus Expressed in Escherichia coli" @default.
- W2369946239 hasPublicationYear "2012" @default.
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