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- W2369947489 abstract "Objective To compare the effect of natural hatching,zona pellucida removal and immunosurgery on mouse blastocyst attachment efficiency,average attachment time and inner cell mass(ICM) cloning efficiency.To observe the mouse blastocyst growth behavior on primary mouse embryonic fibroblast feeder layers (PMEF).Methods Mouse blastocysts were obtained by ovarian hyperstimulation and divided into three groups at random.Group A:natural hatching,group B:zona pellucida removal and group C:trophectoderm cells removal by immunosurgery to take out the ICM.Results Attachment efficiency,ICM cloning efficiency and average attachment time were 83%,83%,36～48h in group A,82%,82%,24～36h in group B and 71%,71%,24～36h in group C respectively.Trophectoderm cells and ICM proliferated together.Trophectoderm cells didn't expand to a wide range and didn't form translucent film like structure on the feeder layers.ICM grew in more rounded clumps before differentiation.Conclusion There is no significant difference between three groups in blastocyst attachment efficiency and ICM cloning efficiency ( P 0.05).The average attachment time is shorter in group B and group C than that in group A." @default.
- W2369947489 created "2016-06-24" @default.
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- W2369947489 date "2004-01-01" @default.
- W2369947489 modified "2023-09-25" @default.
- W2369947489 title "Mouse blastocyst growth behavior on feeder layers" @default.
- W2369947489 hasPublicationYear "2004" @default.
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