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- W2369979747 abstract "Objective:To clone the correct KGF gene and study the factor influencing its expression.Methods:KGF gene was cloned by RT_PCR, and expressed in pBV220.The KGF RNA was analysed by RNAdraw software.Results:The expression of KGF with its signal sequence was about 10%, but 1%-2% without its signal sequence.RNAdraw software showed that the beginning of the RNA of the latter formed the stem_ring structure,while that of the former did not.Conclusions:The expression of KGF gene is very low in E.coli and its signal sequence is a very important factor for its expression." @default.
- W2369979747 created "2016-06-24" @default.
- W2369979747 creator A5046407398 @default.
- W2369979747 date "2003-01-01" @default.
- W2369979747 modified "2023-09-25" @default.
- W2369979747 title "Cloning of human keratinocyte growth factor(KGF) and analysis of its expression in E.coli" @default.
- W2369979747 hasPublicationYear "2003" @default.
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