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- W2369988045 abstract "Objective: To investigate the effect of U2AF65 expression level on alternative splicing of UBQLN1 gene. Methods: Two U2AF65-siRNA vectors were constructed by using the pSR-GFP / Neo vector, and then they were transfected into 293T cells. The effect of RNA interference was examined by methods of Western blotting and QRT-PCR, and the alternative splicing of UBQLN1 was detected through RT-PCR. Results: The expression of U2AF65 was suppressed by U2AF65-siRNA vectors. The result of RT-PCR revealed that knockdown of U2AF65 led to an increase in exon 8 skipping of UBQLN1. Conclusion: U2AF65 may regulate alternative splicing of UBQLN1 through changes of expression level." @default.
- W2369988045 created "2016-06-24" @default.
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- W2369988045 date "2010-01-01" @default.
- W2369988045 modified "2023-09-24" @default.
- W2369988045 title "Changes of U2AF65 Expression Level Affect Alternative Splicing of UBQLN1 Gene" @default.
- W2369988045 hasPublicationYear "2010" @default.
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