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- W2370010714 abstract "Objective To establish a Amplified Fragment Length Polymorphism(AFLP) method for rapid diagnosis of Streptococcus pneumonia.Methods Based on the 16S rRNA nucleic sequence of streptococcus pneumonia,a pair of primers was designed for AFLP.The reaction conditions were optimized,and the specificity,sensitivity,and practicability of AFLP were tested using 7 streptococcus pneumonia strains and 15 non-streptococcus pneumonia strains in blood.Results All the 7 strains of streptococcus pneumonia in blood yielded positive results in AFLP,and the 15 strains of other bacteria in blood all showed negative results.The results of streptococcus pneumonia count showed that AFLP was capable of detecting streptococcus pneumonia at a level as low as 1.5×104cfu/ml in blood.Conclusion The established AFLP method has high specificity and sensitivity for detecting streptococcus pneumonia in blood and is applicable in field of rapid diagnosis of Streptococcus pneumoniae in blood." @default.
- W2370010714 created "2016-06-24" @default.
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- W2370010714 date "2011-01-01" @default.
- W2370010714 modified "2023-09-23" @default.
- W2370010714 title "Establishment and Application of AFLP Method for Rapid Diagnosis of Streptococcus Pneumoniae in Blood" @default.
- W2370010714 hasPublicationYear "2011" @default.
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