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- W2370042960 abstract "To provide an experimental evidence for rapid diagnosis of those pathogenic infection, multiplex-PCR assay was conducted to simultaneously detect Escherichia coli, Salmonella, Staphylococcus aureus, Shigella flexneri and Pseudomonas aeruginosa in soil. According to the toxin genes, the high-conservative genes and specific genes of the pathogenic enterobacterial strains, five primer sets were selected to simultaneously detect the pathogenic enterobacterial strains by multiplex-PCR method. The results showed that the multiplex-PCR using these five primer sets produced specific amplicons of expected sizes and the detection limits for the bacterial targets were estimated at 104cfu · g-1. Multiplex-PCR analysis, applied in analysis of soil samples, could greatly shorten the detection time (only need 3 to 4 hours). It provided a cost-effective way to detect and control the transmission of pathogens and also could be extended and applied to other fields, such as environmental inspection, water detection, food sanitation supervision, commodity inspection and detection, etc." @default.
- W2370042960 created "2016-06-24" @default.
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- W2370042960 date "2013-01-01" @default.
- W2370042960 modified "2023-09-28" @default.
- W2370042960 title "Detection of pathogenic enterobacteria in soil by multiplex-PCR" @default.
- W2370042960 hasPublicationYear "2013" @default.
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