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- W2370082558 abstract "Objective: To evaluate the effect of EDTA and platelet-rich plasma(PRP) on the attachment and proliferation of human periodontal ligament fibroblasts on diseased root surfaces in vitro.Methods: Periodontitis-affected teeth were collected and sectioned with a water-cooled diamond disc to prepare the 4mm×4mm diseased root slices.The diseased root surfaces slices were then scaled.Test slices were exposured to either EDTA(24%,pH=6.7) or PRP.The controls were treated with nothing.Double-sided adhesive tape was used to fix the root slices to the bottom of wells in a 48-well tissue culture plate.The treated and untreated root slices were then sterilized under UV light for 2 hours prior to seeding with cells.The culture were allowed to attach for 24 or 48 hours using standard incubation conditions.The attached and proliferated cells were visualized using MTT staining.Results: The untreated root surfaces demonstrated the least number of attached cells.The root surfaces treated with EDTA or PRP alone all significantly increased cell attachment(P0.05).When PRP were added to previously EDTA-etched root surfaces,cell attachment was further enhanced(P0.01).Diseased root surfaces untreated with EDTA or PRP did not enhance cell proliferation.cell proliferation onto diseased root surfaces treated with EDTA or PRP alone was slightly increased(P0.05).When PRP were added to previously EDTA-etched root surfaces,cell proliferation was also enhanced.(P0.05).Conclusion: Freshly prepared PRP or EDTA could effectively enhance the attachment and proliferation of human periodontal ligament fibroblasts onto human diseased root surfaces." @default.
- W2370082558 created "2016-06-24" @default.
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- W2370082558 date "2009-01-01" @default.
- W2370082558 modified "2023-09-23" @default.
- W2370082558 title "Effects of deminerazation and platelet-rich plasma on periodontal ligament fibroblasts attachment and proliferation on diseased root surfaces" @default.
- W2370082558 hasPublicationYear "2009" @default.
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