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- W2370123143 abstract "Objective To investigate rat hepatocyte apoptosis induced by TNF α in vitro. Methods TNF α induced apoptosis in rat hepatocytes pretreated with transcriptional inhibitors actinomycin (ActD) or D galactosamine (GalN). Hepatocytic apoptosis was detected by light and electron microscope and DNA electrophoresis.Results Cytoplasmic blebs, condensation of chromatin into crescentric caps adjacent to the nuclear membrane were seen in hepatocytes treated with TNF α+ActD or TNF α+GalN: under light or electron microscope. The characteristic “ladder” pattern of DNA fragments was present in agarose gel electrophoresis of DNA isolated from hepatocytes treated with TNF α+ActD. Conclusion TNF α can induce apoptosis of rat hepatocytes in vitro." @default.
- W2370123143 created "2016-06-24" @default.
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- W2370123143 date "2000-01-01" @default.
- W2370123143 modified "2023-10-14" @default.
- W2370123143 title "Effect of rat hepatocyte apoptosis induced by TNF-α in vitro" @default.
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