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- W2370141411 abstract "Objective:To construct mutants of glia cell line derived neurotrophic factor receptor alpha 1 (GFRα1) and to establish PC12 cell lines stably expressing the constructed mutants. Methods: FugGENE 6 transfect reagent was used to construct stably co-transfected PC12 cell lines by the plasmids PC DNA3-rGFRα1 wild type and rGFRα1 mutants respectively with PC DNA3.1-Ret plasmid. Postive cell clones were selected with medium containing G418 and Hygromycin. The expression and location of rGFRα1 and Ret were detected by Western blot and cell immuno-flurescene test respectively.Results: Western blot and cell immuno-fluorescence test showed that rGFRα1 mutants and Ret had been expressed correctly. Conclusion: PC12 cell lines expressing either rGFRα1 or its mutants with RET have been successfully constructed,which provides basis for detecting the key single amino acid of GFRα1 involved in its biological function." @default.
- W2370141411 created "2016-06-24" @default.
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- W2370141411 date "2004-01-01" @default.
- W2370141411 modified "2023-09-23" @default.
- W2370141411 title "Preparation of GFRα1 mutants and establishment of PC12 cell line stably expressing constructed mutants" @default.
- W2370141411 hasPublicationYear "2004" @default.
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