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- W2370142766 abstract "To express the gene of TccP of EHEC O157∶H7 in E.coli and prepare the rabbit antibody against TccP.the gene of Z3072 DNA was amplified from EHEC O157∶H7 by PCR.After confirmed by DNA sequencing,the Z3072-DNA was cloned into expression vector pET-28a(+) and then the Z3072 gene was expressd in E.coli under the IPTG induction.The protein was prmarily purified with the purity of about 80%.The rabbit antibody against TccP was prepared by immunization of two New Zealand white rabbits using the purified TccP as immunogen.The titer and specificity of the antisera were determined by ELISA and Western blot assay.It was demonstrated that the 1 014bp Z3072 gene was amplified,cloned,and expressed in E.coli.The expressed product existed in the bacterial inclusion body.The rabbit antibody against TccP was prepared and its titer was about 1∶640 000.Western blot analysis showed that the antibody cold bind to the expressed TccP protein specifically.In this way,the TccP protein was expressed in E.coli and rabbit against TccP was prepared successfully,thus providing the necessary materials for further study." @default.
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- W2370142766 date "2007-01-01" @default.
- W2370142766 modified "2023-09-23" @default.
- W2370142766 title "Prokaryotic expression of Tir-cytoskeleton coupling protein from EHEC O157 H7 and identification of its antigenicity" @default.
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