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- W2370152514 abstract "Objective An ultrasensitive colorimetric method for detecting protein by nanoparticle-modified aptamer was established. Methods In this assay, target protein was bound to nanoparticle-modified aptamer and another aptamer probe modified with biotion together forming a sandwich-like complex.Thereafter, the sandwich-like complex was captured in microwell by avidin.After amplification by silver-enhancement, the absorbance resulted from aptamer-protein complex was determined by colorimetry. Results In the range of 1 fM-1 μM, the absorbance induced by the concentration of PDGF was dose-dependent, R~2=0.9801. The minimal detection limit was 1fM of targeted protein.Conclusion this new NP-modified aptamer assay could offer an ultrasensitive detection for protein. It requires no complicated or expensive instrument and is easy to use. It has a good future for detecting ultratrace level of disease-related protein markers that can not be detected by conventional methods." @default.
- W2370152514 created "2016-06-24" @default.
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- W2370152514 date "2006-01-01" @default.
- W2370152514 modified "2023-09-28" @default.
- W2370152514 title "Ultrasensitive colorimetric detection of protein by nanoparticle-modified aptamer" @default.
- W2370152514 hasPublicationYear "2006" @default.
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