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- W2370168264 abstract "Objective To analyze the characterization of ultrasound-assisted digestion for in-solution protein sample.MethodsBovine serum albumin was digested by ultrasound-assisted digestion and the digested peptides were analyzed by MALDI-TOF mass spectrometry.After database searching the identified peptides from two methods were analyzed.Results The digestion time of ultrasound-assisted method can be shortened to 10 minutes,but the peptide recovery was 8% lower than that of overnight method.For peptide identifications the MASCOT score,protein coverage and peptide number of ultrasound-assisted method was about 1 fold,10% and 40% higher than that of overnight method,but the miscleavage peptide rate as 22% higher.Analysis of the peptides only identified from ultrasound-assisted method showed that molecular weight distribution was 2~3 ku and most of them were miscleavage peptides.Conclusions Ultrasound-assisted digestion method can significantly shorten protein digestion time and obtain better peptide identifications,but the sample loss will be increased and part of the peptides are not completely digested." @default.
- W2370168264 created "2016-06-24" @default.
- W2370168264 creator A5018302736 @default.
- W2370168264 date "2013-01-01" @default.
- W2370168264 modified "2023-09-26" @default.
- W2370168264 title "Analysis of the characteristics of ultrasound-assisted digestion method for in-solution protein sample" @default.
- W2370168264 hasPublicationYear "2013" @default.
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