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- W2370173824 abstract "To identify and compare predicted the B cell-epitopes between NDV F_ 48 E_9 and La Sota strain, The predicted 4 poly-epitopes genes segments of each strain weve cloned, then were constructed into Prokaryotic vector separately. The expressed 8 recombinant proteins were analysed by SDS-PAGE, molecular weight of four peptides segments P1, P2, P5 and P6 are 8.0kDa, 10.8kDa, 13.5kDa, 10.5kDa, respectively.The Antigenicity of expressed peptides was identified with anti-F48E9 and anti-La Sota strains serum. The result showed that peptides P1, P2, P5 and P6 of NDV F_ 48 E_9 and La Sota could reacted with both anti-serum. However, the Western blot of peptide P5 against anti-F48E9 and anti-La Sota serum showed a strain specific reaction. Then P5 ploypeptide and mChIL-18 protein were purified by Ni-NTA Resin. Using mChIL-18 as adjuvant, immunized SPF chickens with P5 peptide of F48E9 or La Sota,serum were collected and NDV specific antibody titers were detected with ELISA. The results indicate that F48E9 P5 peptide (FP5) in combination with mChIL-18 protein has the highest antibody titer among all immunized groups, and it is the only group that could protect some of immunized chicken from death (20%). Finding of immunogenicity difference between FP5 peptide and LP5 peptide, suggested that there are strain specific epitopes exist in F48E9 and La Sota F protein." @default.
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- W2370173824 date "2006-01-01" @default.
- W2370173824 modified "2023-09-23" @default.
- W2370173824 title "F protein B Cell Epitopes Between Comparison of Immunogenicity of NDV F_(48) E_9 and La Sota Strains" @default.
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