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- W2370213952 abstract "To obtain a full-length cDNA library of Eisenia foetida for the purpose of gene screening and functioning study,total RNA extraction method from Eisenia foetida used is TRIZOL Reagent to construct a cDNA library by the carrier of λTriplex2~(TM) using the SMART cDNA Library Construction Kit.The quality of the constructed full-length cDNA library was evaluated.The primary library showed a titer of 1.3×10~6 pfu/mL of the cDNA insets,and the amplified library has a titer of 1.0×10~8.The recombinant rates were about 99% and the average length of the cDNA inserts was about 1.1 kb.This constructed cDNA full-length library provided an essential resource for the further gene functional studies and gene discoveries of Eisenia foetida." @default.
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- W2370213952 date "2006-01-01" @default.
- W2370213952 modified "2023-09-23" @default.
- W2370213952 title "Construction and primary identification of full-length cDNA library of Eisenia foetida" @default.
- W2370213952 hasPublicationYear "2006" @default.
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