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- W2370239579 abstract "To identify Hemagglutinating encephalomyelitis virus (HEV) 67N receptor in PK cell membrane, polyclonal antibody to HEV was prepared with immunizing rabbits by injecting the purified S1 protein; after SDS-polyacrylamid gel electrophoresis (SDS-PAGE), the PK cell membrane proteins were transferred to nitrocellulose membrane; and virus overlay protein binding assay (VOPBA) was performed by the recombinant S1 protein to identify the receptor protein binding HEV-S1. The results showed that the 1 797 bp S1 gene fragment was obtained by PCR amplification. Identification results by PCR with S1 special primers and AOX1 primers suggested that the recombined yeast expression plasmid was constructed successfully. After the recombined strains were induced by 1% methanol, a molecular weight of 73 kD in medium supernatant could be specifically recognized by polyclonic antibody against HEV. After transmembrane, the PK cell membrane proteins were bound with the purified S1 protein, and one clear protein band of 90 kD was found by DAB colouration." @default.
- W2370239579 created "2016-06-24" @default.
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- W2370239579 date "2008-01-01" @default.
- W2370239579 modified "2023-09-28" @default.
- W2370239579 title "Identification of Hemagglutinating encephalomyelitis virus Receptor in PK Cells Membrane" @default.
- W2370239579 hasPublicationYear "2008" @default.
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