Matches in SemOpenAlex for { <https://semopenalex.org/work/W2370293616> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2370293616 endingPage "51" @default.
- W2370293616 startingPage "148" @default.
- W2370293616 abstract "To construct the eukaryotic expression plasmid containing mouse vasoactive intestinal peptide(VIP) gene with biological activities.VIP cDNA including the sequences of signal peptide was cloned from mouse thymus by RT-PCR, and then inserted into the mammalian expression vector pcDNA3.1 between Hind III and EcoR I restriction sites. COS-7 cells were transfected with pcDNA3. 1-VIP using liposome, the expression of VIP was identified by Western blot and ELISA. Supernatant of transfected cell culture was added to LPS-stimulated macrophages and the TNF-alpha production in cell medium was observed by ELISA.The cloned VIP cDNA was confirmed by enzyme digestion and DNA sequencing. The expression of VIP was detected in the pcDNA3. 1-VIP transfected COS-7 cells by Western blot and ELISA. The VIP in culture supernatant potently inhibited TNF-alpha production by LPS-induced Macrophages in vitro.The eukaryotic expression plasmid that expresses biological active murine VIP has been constructed successfully." @default.
- W2370293616 created "2016-06-24" @default.
- W2370293616 creator A5005478652 @default.
- W2370293616 creator A5018484014 @default.
- W2370293616 creator A5068098264 @default.
- W2370293616 creator A5069482637 @default.
- W2370293616 date "2005-03-01" @default.
- W2370293616 modified "2023-09-23" @default.
- W2370293616 title "[Construction of recombinant plasmid containing mouse vasoactive intestinal peptide gene and its expression in COS-7 cells]." @default.
- W2370293616 doi "https://doi.org/10.3785/j.issn.1008-9292.2005.02.011" @default.
- W2370293616 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15812889" @default.
- W2370293616 hasPublicationYear "2005" @default.
- W2370293616 type Work @default.
- W2370293616 sameAs 2370293616 @default.
- W2370293616 citedByCount "1" @default.
- W2370293616 countsByYear W23702936162018 @default.
- W2370293616 crossrefType "journal-article" @default.
- W2370293616 hasAuthorship W2370293616A5005478652 @default.
- W2370293616 hasAuthorship W2370293616A5018484014 @default.
- W2370293616 hasAuthorship W2370293616A5068098264 @default.
- W2370293616 hasAuthorship W2370293616A5069482637 @default.
- W2370293616 hasConcept C104317684 @default.
- W2370293616 hasConcept C118303440 @default.
- W2370293616 hasConcept C150194340 @default.
- W2370293616 hasConcept C153911025 @default.
- W2370293616 hasConcept C170493617 @default.
- W2370293616 hasConcept C187882448 @default.
- W2370293616 hasConcept C22744801 @default.
- W2370293616 hasConcept C2776415932 @default.
- W2370293616 hasConcept C33161422 @default.
- W2370293616 hasConcept C40767141 @default.
- W2370293616 hasConcept C54009773 @default.
- W2370293616 hasConcept C54355233 @default.
- W2370293616 hasConcept C55493867 @default.
- W2370293616 hasConcept C81885089 @default.
- W2370293616 hasConcept C8330315 @default.
- W2370293616 hasConcept C86803240 @default.
- W2370293616 hasConceptScore W2370293616C104317684 @default.
- W2370293616 hasConceptScore W2370293616C118303440 @default.
- W2370293616 hasConceptScore W2370293616C150194340 @default.
- W2370293616 hasConceptScore W2370293616C153911025 @default.
- W2370293616 hasConceptScore W2370293616C170493617 @default.
- W2370293616 hasConceptScore W2370293616C187882448 @default.
- W2370293616 hasConceptScore W2370293616C22744801 @default.
- W2370293616 hasConceptScore W2370293616C2776415932 @default.
- W2370293616 hasConceptScore W2370293616C33161422 @default.
- W2370293616 hasConceptScore W2370293616C40767141 @default.
- W2370293616 hasConceptScore W2370293616C54009773 @default.
- W2370293616 hasConceptScore W2370293616C54355233 @default.
- W2370293616 hasConceptScore W2370293616C55493867 @default.
- W2370293616 hasConceptScore W2370293616C81885089 @default.
- W2370293616 hasConceptScore W2370293616C8330315 @default.
- W2370293616 hasConceptScore W2370293616C86803240 @default.
- W2370293616 hasIssue "2" @default.
- W2370293616 hasLocation W23702936161 @default.
- W2370293616 hasOpenAccess W2370293616 @default.
- W2370293616 hasPrimaryLocation W23702936161 @default.
- W2370293616 hasRelatedWork W2353613679 @default.
- W2370293616 hasRelatedWork W2356503925 @default.
- W2370293616 hasRelatedWork W2357410309 @default.
- W2370293616 hasRelatedWork W2359348659 @default.
- W2370293616 hasRelatedWork W2361639863 @default.
- W2370293616 hasRelatedWork W2363386379 @default.
- W2370293616 hasRelatedWork W2368809917 @default.
- W2370293616 hasRelatedWork W2369227601 @default.
- W2370293616 hasRelatedWork W2373100251 @default.
- W2370293616 hasRelatedWork W2373690171 @default.
- W2370293616 hasRelatedWork W2375148766 @default.
- W2370293616 hasRelatedWork W2376279029 @default.
- W2370293616 hasRelatedWork W2376869834 @default.
- W2370293616 hasRelatedWork W2380046028 @default.
- W2370293616 hasRelatedWork W2382896905 @default.
- W2370293616 hasRelatedWork W2383434177 @default.
- W2370293616 hasRelatedWork W2388367005 @default.
- W2370293616 hasRelatedWork W2468625006 @default.
- W2370293616 hasRelatedWork W3145787805 @default.
- W2370293616 hasRelatedWork W1839405733 @default.
- W2370293616 hasVolume "34" @default.
- W2370293616 isParatext "false" @default.
- W2370293616 isRetracted "false" @default.
- W2370293616 magId "2370293616" @default.
- W2370293616 workType "article" @default.