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- W2370298310 abstract "Objective:To develop a HPLC quantitative method for the determination of chlorgenic acid and baicalin in Yinhuang Oral liquid.Methods: An Agilent C18 column(ZORBAX Eclipse XDB-C18,4.6×150 mm,5 μm)was used and a mixture of methanol-water-phosphorc acid(50:950:5)was used as mobile phase A,methanol as mobile phase B,gradient elution:0～30,A:95%～35%,B:5%～65%.The flow rate was 1.0 mL/min and monitored at 327 nm.Results: The retention time of chlorgenic acid and baicalin were 10.5 min and 23.8 min,respectively.The regress equation for chlorgenic acid was Y=0.032 00X-0.021 60,r=0.999 9,and the linear range was 5.040～45.36 μg/mL.Baicalin was Y=0.049 70X-0.488 0,r=0.999 9 and the linear range was 44.88～403.9 μg/mL.The average recovery of chlorgenic acid and baicalin were(n=6)95.7% and 99.0%;RSD 0.43% and 0.33% respectively.Conclusion: Between the determination of this method in CP 2005,this method was sensiteve,time-saving and accurate." @default.
- W2370298310 created "2016-06-24" @default.
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- W2370298310 date "2008-01-01" @default.
- W2370298310 modified "2023-09-25" @default.
- W2370298310 title "Simultaneous determination of chlorogenic acid and baicalin in Yinhuang oral liquid by HPLC" @default.
- W2370298310 hasPublicationYear "2008" @default.
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