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- W2370307851 abstract "Objective To observe the anti-hepatitis B and anti-hepatoma effect of different extracts in serums of Hypericum japonicum Thunb.in vitro.Methods Enzyme linked immune response was used to investigate the effect of the different extracts in serums of Hypericum japonicum Thunb.on HBeAg and HBsAg excreted by 2215 cell in vitro.MTT technique was used to investigate the effect of the different extracts in serums of Hypericum japonicum Thunb.on the growth of hepatoma BEL-7404 cell in vitro.Results EtOH and BuOH extracts showed better inhibitory rates on HBeAg and HBsAg as 34.84%,57.95% and 76.02%,53.15%(P0.05)in 5% serum respectively.EtOAc extracts showed better inhibitory rates on HBeAg and HBsAg as 79.79% and 57.22%(P0.05)in 20% serum respectively.H2O extracts had stable inhibitory rates on HBeAg and HBsAg in the three different concentration of serum and the inhibitory rates were all more than 70% and 30% respectively.All extracts showed the inhibitory activity on the growth of hepatoma BEL-7404 cell in vitro and there was obvious dose-effect relationship.The inhibitory rates of EtOH,EtOAc,BuOH and H2O extracts in 20% serum were 29.74%,53.80%,40.79% and 54.24%,respectively(P0.01).Conclusion The different extracts of Hypericum japonicum Thunb.in serum have the inhibitory effect on HBeAg and HBsAg excreted by 2215 cell and on the growth of hepatoma BEL-7404 cell in vitro.The stable active compounds of anti-Hepatitis B mainly distribute in H2O extracts and the active compounds of anti-hepatoma evenly distribute in each extracts." @default.
- W2370307851 created "2016-06-24" @default.
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- W2370307851 date "2009-01-01" @default.
- W2370307851 modified "2023-09-27" @default.
- W2370307851 title "Experimental Study on Anti-hepatitis B and Anti-hepatoma Effect of Different Extracts in Serum of Hypericum japonicum Thunb.in Vitro" @default.
- W2370307851 hasPublicationYear "2009" @default.
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