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- W2370322385 abstract "Objective To explore the protective mechanism of ischemic preconditioning (IP) on the lung isehemia-reperfusion injury (IRI).Methods Thirty SD rats were randomly divided into precon- ditioning group (group I),control group (group C) and sham operation group (group S) (10 animals in each group).Rat lungs in group I received IP and the following isehemia-reperfusion insults,while those in group C suffered ischemia-reperfusion insults only.The total proteins of those lung samples were separated by means of immobilized pH gradient-based two-dimensional gel electrophoresis (2-DE).The differently expressed spots were identified by matrix-assisted laser desorption/ionization time of flight mass spectrome- try and database searching.Rats in group S were not administered with any other intervention after chest incision and lobe dissection.Western blot was employed to compare the HSP27 expression in lung tissues among the groups.Results The study has established the well-resolved,reproducible 2-DE reference map of iscbemia-reperfusion injury in rat lung tissue.Forty-five protein spots were differentially expressed and their PMF data showed that 30 proteins could be matched to known protein sequences and unambiguously identified.Western Blot showed that heat shock protein 27 was up-regulated during the classic precondi- tioning.Conclusion The HSP27 plays a vital role in the protective procession of iscbemie preconditioning on lung IRI." @default.
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- W2370322385 date "2007-01-01" @default.
- W2370322385 modified "2023-09-26" @default.
- W2370322385 title "Protective mechanism of ischemic preconditioning on the lung ischemia-repurfusion injury in rats" @default.
- W2370322385 hasPublicationYear "2007" @default.
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