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- W2370327928 abstract "Objective To estimate the capability of immuno bl otting(IB) detection of extractable nuclear antigen (ENA) polypeptide autoantibo dy.Methods Detection of anti-U1RNP antibodies in ENA aut oantibody as study object,detection was carried out both in immunoblotting techn ique (IB) and immunodiffusion (ID).The very specific ID detection result was com pared.The coincidence rate of IB was counted.Results Ther e were seven different protein combinations with immunoblotting detection U1RNP antibodies.Protein zones of 73 kDa,32 kDa and 17.5 kDa appeared simultaneity,and the coincidence rate (84.1%) with ID was the maximun.While the 32 kDa one with ID was the minimun.The defference between these groups was the most obvious (P 0.01).Conclusion It is lack of trustiness to detect E NA autoantibody by using IB singly.To enhance the indexes of sensitivity and spe cific,it is essential be combined with other methods." @default.
- W2370327928 created "2016-06-24" @default.
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- W2370327928 date "2005-01-01" @default.
- W2370327928 modified "2023-09-26" @default.
- W2370327928 title "Estimate of ENA Polypeptide Autoantibody Detection Using I mmunoblotting Technique" @default.
- W2370327928 hasPublicationYear "2005" @default.
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