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- W2370367251 abstract "In order to study the effect of different test materials on the detected results of avian leukosis virus(ALV)-p27 antigen by ELISA,the cloacal swabs ALV-p27 antigen were detected by ELISA in 180-day local breeds hen,and the parts of the positive and negative chicken was selected to group test,the serum,egg whites and cell supernatant ALV-p27 antigen were detected by ELISA,the specific genes of ALV were detected by RT-PCR in blood.The results showed that serum,egg white and cell supernatant as ELISA test materials,the positive rate lower of than cloacal swabs,and serum ALV-p27 positive samples include all egg whites and cell supernatant positive samples in positive group.It was a significant correlation between ELISAs with serum and cell supernatant(linear equation:Y=1.8439X-0.1469,the correl was 0.937).In positive group,ALV-p27 gene positive rate lower than cloacal swabs ELISA,but higher than the serum,egg and cell culture medium,and ALV-p27 gene positive samples include serum positive samples by ELISA.ALV-J gp85 gene positive rate of 29.17%,and all positive samples were included in the serum ALV-p27 positive samples.The results suggested that the cloacal swabs as test material may occur false positive results,and egg whites and cell supernatant may occur undetected by ELISA ALV-p27 antigen assay in adult chicken,serum as test material in ELISA could more accurately reflect the state of adult chickens infected with ALV." @default.
- W2370367251 created "2016-06-24" @default.
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- W2370367251 date "2015-01-01" @default.
- W2370367251 modified "2023-09-27" @default.
- W2370367251 title "Effect of Different Test Materials on the Detected Results of Avian Leukosis Virus-p27 Antigen by ELISA" @default.
- W2370367251 hasPublicationYear "2015" @default.
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