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- W2370377131 abstract "Objective To clone and determine the nucleotide sequence of themature parasite infected erythrocyte surface antigen (MESA) gene of Plasmodium falciparum isolate FCC1/HN, and analyze the structure of MESA gene. Methods Four pairs of primers were designed according to the known sequence of MESA gene of Plasmodium falciparum isolate Palo alto. Four overlapped MESA gene fragments were amplified from genomic DNA of P. falciparum isolate FCC1/HN and cloned into pMD18 T vector by T A cloning method.The four DNA fragments were sequenced by dideoxy chain termination method to obtain the full length sequence of MESA gene. The AnthProt and DNAstar software were used to analyze the structure of MESA gene of P. falciparum isolate FCC1/HN and homology comparison of MESA antigens of P. falciparum isolates FCC1/HN, Palo alto and D10. Results Four partial MESA genes were specifically amplified from genomic DNA of P. falciparum isolate FCC1/HN and cloned into pMD18 T vector. Recombinant plasmids containing MESA gene fragments were identified by agarose gel electrophoresis, endonuclease digestion and PCR amplification. The MESA gene of isolate FCC1/HN was 4 102 base pairs in length, with 72.11 %A+T and 27.89 %G+C. The gene contains one intron and is encoding a protein with 1 323 amino acid residues and about 154 470u in molecular weight. The MESA antigens of P. falciparum isolate FCC1/HN, Palo alto and D10 exhibited length and amino acid residue polymorphism. The sequence diversities of MESA antigens of these P. falciparum isolates were mainly located in the repeat block 1, 3, 4, 5 and 7. The results of multiple parameters analysis showed that there were 7 potent antigenic sequential epitopes in the MESA antigen of P. falciparum isolate FCC1/HN. Conclusion The MESA gene of P. falciparum isolates FCC1/HN was cloned and DNA sequence was analyzed. Amino acid residues polymorphism in the MESA antigens of P. falciparum isolates FCC1/HN, Palo alto and D10 were found." @default.
- W2370377131 created "2016-06-24" @default.
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- W2370377131 date "2004-01-01" @default.
- W2370377131 modified "2023-09-25" @default.
- W2370377131 title "Cloning and DNA Sequencing of MESA Gene of Plasmodium falciparum Isolate FCC1/HN" @default.
- W2370377131 hasPublicationYear "2004" @default.
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