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- W2370396985 abstract "The optimal conditions were studied for large scale production of Schistosoma japonicum 28 kDa glutathione S-transferase(Sj28GST) by fermenting recombinant E.coli TB1 in a bioreactor containing the MBL medium(glucose 5 g/L,tryptone 20 g/L,yeast extract 10 g/L KH2PO4 3.5 g/L,K2HPO4 5 g/L,(NH4)2HPO4 3.5 g/L,NaCl 5 g/L and MgSO4·7H2O 1 g/L).These conditions included incubation temperature,pH,inoculation volume,dissolved oxygen concentration,IPTG concentration and start time of IPTG induction.The highest yields of bacterial density(13 OD) and protein concentration(74.32 mg/L) were obtained at incubation temperature of 37 ℃,pH 7.2,dissolved oxygen concentration 20%,0.7 mmol IPTG and start of induction at 5 h post inoculation." @default.
- W2370396985 created "2016-06-24" @default.
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- W2370396985 date "2009-01-01" @default.
- W2370396985 modified "2023-09-24" @default.
- W2370396985 title "STUDIES ON THE FERMENTATION PROCESS OF SJ28GST IN THE RECOMBINANT E.COLI TB1 WITH FERMENTOR" @default.
- W2370396985 hasPublicationYear "2009" @default.
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