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- W2370397273 abstract "Objective: Our aim was to construct and express a recombinant cloning vector bearing the chemically synthesized cDNA of the recombinant IN 1 single chain antibody. Methods: Thirty five segments with the length ranging from 40 to 50 bp were assembled in only one step by using a method of polymerase chain reaction. The entire gene was cloned into pUC18 plasmid. Determined by auto sequencing, the target gene was cloned into prokaryotic expression vector pET28a(+)in fusion form and transformed into E.coli BL21. Results: Sequence analysis showed that the sequence in the gene was almost correct except one base. SDS PAGE analysis showed that a new protein band with molecule weight of 31kd appeared as the expected size. Conclusion: The successful construction and expression of the recombinant vector pET 28a(+) bearing the cDNA might provide material for the further research about the recombinant IN 1 single chain antibody." @default.
- W2370397273 created "2016-06-24" @default.
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- W2370397273 date "2002-01-01" @default.
- W2370397273 modified "2023-09-26" @default.
- W2370397273 title "Construction and Fusion Expression of Recombinant IN-1 Single Chain Antibody Expression Vector" @default.
- W2370397273 hasPublicationYear "2002" @default.
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