Matches in SemOpenAlex for { <https://semopenalex.org/work/W2370413243> ?p ?o ?g. }
Showing items 1 to 59 of
59
with 100 items per page.
- W2370413243 abstract "Human interleukin-2 gene was transferred into human erythroleukemic cell line K562 by retroviral vector. Integration of the foreign gene was confirmed by PCR amplification with neo gene. Using MTT method and IL-2-dependent cell line CTLL-2, IL-2 specific activity was detected as 5-10 units/ 106 cells/24hr in the supernatant of the transduced K562 cell culture. The IL-2 gene-modified K562 celis showed striking morphological changes with their increased celi size. Condensation and margination of chromatin along with cytoplasmic vacuolization and blebbing followed by nuclear lobulation were remarkable under electron microscope while mitbtic figures were very rare. A typical DNA ladder from transduced celis was found in electrophoresis. The results suggested that apoptosis in the transduced K562 cells would be induced by IL-2 gene transfer. Other than the drastic morphological changes, the immunophenotypic change might be involved as well in the celis fonvards apoptosis which prob-ably prevented the IL-2 gene-modified K562 celis from being recognized by LAK cells in our experiments. The transduced celis showed low susceptibility to LAK activity." @default.
- W2370413243 created "2016-06-24" @default.
- W2370413243 creator A5004942213 @default.
- W2370413243 date "1995-01-01" @default.
- W2370413243 modified "2023-09-25" @default.
- W2370413243 title "APOPTOSIS OF K562 CELLS TRANSDUCED WITH IL-2 GENE" @default.
- W2370413243 hasPublicationYear "1995" @default.
- W2370413243 type Work @default.
- W2370413243 sameAs 2370413243 @default.
- W2370413243 citedByCount "0" @default.
- W2370413243 crossrefType "journal-article" @default.
- W2370413243 hasAuthorship W2370413243A5004942213 @default.
- W2370413243 hasConcept C104317684 @default.
- W2370413243 hasConcept C111599444 @default.
- W2370413243 hasConcept C153911025 @default.
- W2370413243 hasConcept C171122931 @default.
- W2370413243 hasConcept C190283241 @default.
- W2370413243 hasConcept C502942594 @default.
- W2370413243 hasConcept C54355233 @default.
- W2370413243 hasConcept C81885089 @default.
- W2370413243 hasConcept C86803240 @default.
- W2370413243 hasConcept C95444343 @default.
- W2370413243 hasConceptScore W2370413243C104317684 @default.
- W2370413243 hasConceptScore W2370413243C111599444 @default.
- W2370413243 hasConceptScore W2370413243C153911025 @default.
- W2370413243 hasConceptScore W2370413243C171122931 @default.
- W2370413243 hasConceptScore W2370413243C190283241 @default.
- W2370413243 hasConceptScore W2370413243C502942594 @default.
- W2370413243 hasConceptScore W2370413243C54355233 @default.
- W2370413243 hasConceptScore W2370413243C81885089 @default.
- W2370413243 hasConceptScore W2370413243C86803240 @default.
- W2370413243 hasConceptScore W2370413243C95444343 @default.
- W2370413243 hasLocation W23704132431 @default.
- W2370413243 hasOpenAccess W2370413243 @default.
- W2370413243 hasPrimaryLocation W23704132431 @default.
- W2370413243 hasRelatedWork W1127386832 @default.
- W2370413243 hasRelatedWork W146484131 @default.
- W2370413243 hasRelatedWork W1896734497 @default.
- W2370413243 hasRelatedWork W2055466727 @default.
- W2370413243 hasRelatedWork W2061026515 @default.
- W2370413243 hasRelatedWork W2086548730 @default.
- W2370413243 hasRelatedWork W2159702108 @default.
- W2370413243 hasRelatedWork W2359500228 @default.
- W2370413243 hasRelatedWork W2361701910 @default.
- W2370413243 hasRelatedWork W2372356089 @default.
- W2370413243 hasRelatedWork W2372807269 @default.
- W2370413243 hasRelatedWork W2372917556 @default.
- W2370413243 hasRelatedWork W2380732903 @default.
- W2370413243 hasRelatedWork W2385198394 @default.
- W2370413243 hasRelatedWork W2386117990 @default.
- W2370413243 hasRelatedWork W2386608908 @default.
- W2370413243 hasRelatedWork W2390187805 @default.
- W2370413243 hasRelatedWork W2392321135 @default.
- W2370413243 hasRelatedWork W2428758719 @default.
- W2370413243 hasRelatedWork W2528930117 @default.
- W2370413243 isParatext "false" @default.
- W2370413243 isRetracted "false" @default.
- W2370413243 magId "2370413243" @default.
- W2370413243 workType "article" @default.