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- W2370421750 abstract "Objective:To investigated the inhibitory effect of B7-2 on pulmonary metastasis by B7-2 gene transfection into breast cancer cells.Methods:A pair of PCR inter primers for B7-2 KpnⅠ was designed based on the sequence registered in GenBank.XbaⅠand KpnⅠ sites were introduced into the 5′ ends of the primers,respectively.Adequate parts out of each end of B7-2 gene in genome were taken as a pair of outer primers.Mononuclear cells were isolated from human peripheral blood,incubated until clustering.Reverse transcript total RNA that isolated from those clustering cells.B7-2 gene was amplified with outer and inner primers with PCR.The PCR fragments were digested with the above two enzymes and then cloned into pcDNA3.1(+) vector to construct recombinant plasmid called B7-2pcDNA.The correct sequencing B7-2pcDNA clone was amplified and transtected into MD-MBA-231 cells.Another group of MD-MBA-231 cells were transfected with idling pcDNA3.1(+).Both transfected groups and the control group were marked with FITC anti-human B7-2 antibody and then detected by flow cytometric analysis.MD-MBA-231/B7,MD-MBA-231/PCDNA3.1,and MD-MBA-231 cells were inoculated into BALB/c mice respectively.The influence of B7-2 on the tumorous metastasis was also observed.Results:The B7-2 recombinant gene was constructed and its length was 952 bp.The B7-2 coding sequence within it was 100% homology with B7-2 homo gene previously registered in GenBank.The flow cytometric analysis shew that B7-2pcDNA group was 36.77% and 51.88% higher than pcDNA3.1(+) and control ones in B7-2 express rate,respectively.The tumor growth rate of MD-MBA-231/B7 xenograft was significantly slower than MD-MBA-231 xenograft in BALB/c mice.MD-MBA-231/B7 cells in orthotopic implantation developed significantly less pulmonary metastasis than MD-MBA-231/pcDNA3.1 and MD-MBA-231 cells.Histologic findings showed that leukocytes were intensively infiltrated in both the MD-MBA-231/B7-2 tumors and its metastatic lesions,however,were scarcely observed in the lesions associated with MD-MBA-231 and MD-MBA-231/pcDNA3.1 cells.Conclusion:B7-2 may play an important role in inhibiting lymph node metastasis by the mechanism of enhanced immunogenicity,and that B7-2 gene transduction might be effective against lymph node metastases of breast cancer." @default.
- W2370421750 created "2016-06-24" @default.
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- W2370421750 date "2007-01-01" @default.
- W2370421750 modified "2023-09-23" @default.
- W2370421750 title "Experimental study on inhibiting growth and metastasis in human breast cancer by B7-2 gene transfection" @default.
- W2370421750 hasPublicationYear "2007" @default.
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