Matches in SemOpenAlex for { <https://semopenalex.org/work/W2370458488> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W2370458488 abstract "A pair of primer sets cqubs01/cquba01 was selected from the primers which were designed based on the specific gene sequences rDNA-ITS and BxPe12 of the pine wood nematode (Bursaphelenchus xylophilus). After optimization, a stable, sensitive (The detection limition was 100 pg/μL with the genome DNA of B. xylophilus as template) and specific PCR detection method was established. Using the specific primers, the 196 bp amplified fragments, which were not homologous with any nematodes but B. xylophilus, were obtained and purified. The product was then ligated with the pMD18-T vector and transformed into Escherichia coli JM109. The selected positive clones were sequenced and those validated were used as a harmless positive control. Nearly 100 samples of 12 batches collected from different areas were detected with this PCR system and the anticipated results were obtained. Fig 3, Tab 2, Ref 8" @default.
- W2370458488 created "2016-06-24" @default.
- W2370458488 creator A5062766009 @default.
- W2370458488 date "2008-01-01" @default.
- W2370458488 modified "2023-09-25" @default.
- W2370458488 title "Establishment of Standard Positive Control and PCR Detection System for Bursaphelenchus xylophilus" @default.
- W2370458488 hasPublicationYear "2008" @default.
- W2370458488 type Work @default.
- W2370458488 sameAs 2370458488 @default.
- W2370458488 citedByCount "0" @default.
- W2370458488 crossrefType "journal-article" @default.
- W2370458488 hasAuthorship W2370458488A5062766009 @default.
- W2370458488 hasConcept C104317684 @default.
- W2370458488 hasConcept C141231307 @default.
- W2370458488 hasConcept C153911025 @default.
- W2370458488 hasConcept C178790620 @default.
- W2370458488 hasConcept C185592680 @default.
- W2370458488 hasConcept C18903297 @default.
- W2370458488 hasConcept C2776857380 @default.
- W2370458488 hasConcept C2777563447 @default.
- W2370458488 hasConcept C2778830712 @default.
- W2370458488 hasConcept C2779442720 @default.
- W2370458488 hasConcept C3018223157 @default.
- W2370458488 hasConcept C49105822 @default.
- W2370458488 hasConcept C54355233 @default.
- W2370458488 hasConcept C556039675 @default.
- W2370458488 hasConcept C71924100 @default.
- W2370458488 hasConcept C86803240 @default.
- W2370458488 hasConceptScore W2370458488C104317684 @default.
- W2370458488 hasConceptScore W2370458488C141231307 @default.
- W2370458488 hasConceptScore W2370458488C153911025 @default.
- W2370458488 hasConceptScore W2370458488C178790620 @default.
- W2370458488 hasConceptScore W2370458488C185592680 @default.
- W2370458488 hasConceptScore W2370458488C18903297 @default.
- W2370458488 hasConceptScore W2370458488C2776857380 @default.
- W2370458488 hasConceptScore W2370458488C2777563447 @default.
- W2370458488 hasConceptScore W2370458488C2778830712 @default.
- W2370458488 hasConceptScore W2370458488C2779442720 @default.
- W2370458488 hasConceptScore W2370458488C3018223157 @default.
- W2370458488 hasConceptScore W2370458488C49105822 @default.
- W2370458488 hasConceptScore W2370458488C54355233 @default.
- W2370458488 hasConceptScore W2370458488C556039675 @default.
- W2370458488 hasConceptScore W2370458488C71924100 @default.
- W2370458488 hasConceptScore W2370458488C86803240 @default.
- W2370458488 hasLocation W23704584881 @default.
- W2370458488 hasOpenAccess W2370458488 @default.
- W2370458488 hasPrimaryLocation W23704584881 @default.
- W2370458488 hasRelatedWork W1505085116 @default.
- W2370458488 hasRelatedWork W1971097625 @default.
- W2370458488 hasRelatedWork W1971786454 @default.
- W2370458488 hasRelatedWork W1995571206 @default.
- W2370458488 hasRelatedWork W2012395489 @default.
- W2370458488 hasRelatedWork W2015896015 @default.
- W2370458488 hasRelatedWork W2060571288 @default.
- W2370458488 hasRelatedWork W2074882042 @default.
- W2370458488 hasRelatedWork W2126562638 @default.
- W2370458488 hasRelatedWork W2140306697 @default.
- W2370458488 hasRelatedWork W2370786920 @default.
- W2370458488 hasRelatedWork W2371048480 @default.
- W2370458488 hasRelatedWork W2377489031 @default.
- W2370458488 hasRelatedWork W2378358534 @default.
- W2370458488 hasRelatedWork W2392569295 @default.
- W2370458488 hasRelatedWork W2612345743 @default.
- W2370458488 hasRelatedWork W2937578266 @default.
- W2370458488 hasRelatedWork W2946818352 @default.
- W2370458488 hasRelatedWork W344269784 @default.
- W2370458488 hasRelatedWork W3020258550 @default.
- W2370458488 isParatext "false" @default.
- W2370458488 isRetracted "false" @default.
- W2370458488 magId "2370458488" @default.
- W2370458488 workType "article" @default.