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- W2370479657 abstract "Objective To screen anti-connective tissue growth factor(CTGF) human single-chain variable fragment(ScFv) from human phage antibody library,prokaryotic expression and to identify its activity.Methods The human phage antibody library was screened by using rhCTGF as the target,and the positive clones were selected by phage-ELISA to induce its prokaryotic expression and to purify the protein,and the high purity ScFv protein was obtained.Its activity was identified by ELISA and Millicell migration assay,respectively.Results After three rounds of screening,only 1 clone,designated 1C5,with good reactivity to rhCTGF was selected.1C5 was found to bind human CTGF specifically in ELISA,and inhibit CTGF's function in Millicell migration assay,indicating its neutralizing activity.Conclusion An anti-CTGF human single-chain Fv clone was successfully identified,which could be used as therapeutic agent candidate for CTGF-related disease." @default.
- W2370479657 created "2016-06-24" @default.
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- W2370479657 date "2013-01-01" @default.
- W2370479657 modified "2023-09-23" @default.
- W2370479657 title "Screening,expression and activity identification of anti-CTGF human single-chain Fv" @default.
- W2370479657 hasPublicationYear "2013" @default.
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