Matches in SemOpenAlex for { <https://semopenalex.org/work/W2370487248> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2370487248 endingPage "20" @default.
- W2370487248 startingPage "16" @default.
- W2370487248 abstract "The latent membrane protein gene (LMP) of Epstein-Barr virus (EBV) was thought to play an important role in the carcinogenesis of nasopharyngeal carcinoma (NPC). In this study, the authors investigated the effects of antisense RNA (AsRNA) on LMP for down regulating at the target gene over expression in EBV transformed lymphoid cells, and set up an antisense system to inhibit LMP expression.Constructing the single strand antisense transcription system in vitro, the authors have got large amount of AsRNA. Designing and setting up an antisense tracing system in situ (ATSIS), the authors could observe the living particles of AsRNA/sense RNA duplex dimer. With time lapse phase-contrast microscopy, the agglutination phenotype on living cells was easily detected by MTT test, the inhibition rate on EBV transformed cells was calculated.LMP 1.9 fragment ligated into pGEM vector in reverse orientation have been constructed and produced a plentiful amount of AsLMPmRNA which could incorporated into both B95-8 and C1936 cell lines by endophagocytosis and formed the duplex dimer of As/Sense RNA. This particles have been visualized in situ when labelling 35S isotope by ATSIS. When AsLMPmRNA acted as agents for specific inhibition to LMP over expression, the transform phenotype of cell agglutination have been suppressed and MTT particle formatin was apparently reduced both two EBV tansformed cell lines.AsLMPmRNA targets at sense strand have a high effectiveness of down-regulation on EBV-LMP overexpression. This down regulating function of LMP and growth inhibition on transformed cell is demonstrated by the antisenes tracing system in situ (ATSIS). The results provide a clue to overcome the latent EBV infection in human bodies all living long time and to prevent it inducing NPC in high incidence area by antisense strategies." @default.
- W2370487248 created "2016-06-24" @default.
- W2370487248 creator A5003672032 @default.
- W2370487248 creator A5005005836 @default.
- W2370487248 creator A5013285911 @default.
- W2370487248 creator A5014236405 @default.
- W2370487248 creator A5022059483 @default.
- W2370487248 creator A5038219423 @default.
- W2370487248 creator A5052274720 @default.
- W2370487248 creator A5072570482 @default.
- W2370487248 creator A5077008845 @default.
- W2370487248 date "2002-01-01" @default.
- W2370487248 modified "2023-10-14" @default.
- W2370487248 title "[Inhibiting target gene expression and controlling growth of Epstein-Barr virus transformed cells by antisense RNA transcripts]." @default.
- W2370487248 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12500390" @default.
- W2370487248 hasPublicationYear "2002" @default.
- W2370487248 type Work @default.
- W2370487248 sameAs 2370487248 @default.
- W2370487248 citedByCount "0" @default.
- W2370487248 crossrefType "journal-article" @default.
- W2370487248 hasAuthorship W2370487248A5003672032 @default.
- W2370487248 hasAuthorship W2370487248A5005005836 @default.
- W2370487248 hasAuthorship W2370487248A5013285911 @default.
- W2370487248 hasAuthorship W2370487248A5014236405 @default.
- W2370487248 hasAuthorship W2370487248A5022059483 @default.
- W2370487248 hasAuthorship W2370487248A5038219423 @default.
- W2370487248 hasAuthorship W2370487248A5052274720 @default.
- W2370487248 hasAuthorship W2370487248A5072570482 @default.
- W2370487248 hasAuthorship W2370487248A5077008845 @default.
- W2370487248 hasConcept C104317684 @default.
- W2370487248 hasConcept C138885662 @default.
- W2370487248 hasConcept C143141573 @default.
- W2370487248 hasConcept C147789679 @default.
- W2370487248 hasConcept C150194340 @default.
- W2370487248 hasConcept C153911025 @default.
- W2370487248 hasConcept C179926584 @default.
- W2370487248 hasConcept C185592680 @default.
- W2370487248 hasConcept C202416437 @default.
- W2370487248 hasConcept C41895202 @default.
- W2370487248 hasConcept C54355233 @default.
- W2370487248 hasConcept C55493867 @default.
- W2370487248 hasConcept C555283112 @default.
- W2370487248 hasConcept C67705224 @default.
- W2370487248 hasConcept C81885089 @default.
- W2370487248 hasConcept C86803240 @default.
- W2370487248 hasConcept C95911042 @default.
- W2370487248 hasConceptScore W2370487248C104317684 @default.
- W2370487248 hasConceptScore W2370487248C138885662 @default.
- W2370487248 hasConceptScore W2370487248C143141573 @default.
- W2370487248 hasConceptScore W2370487248C147789679 @default.
- W2370487248 hasConceptScore W2370487248C150194340 @default.
- W2370487248 hasConceptScore W2370487248C153911025 @default.
- W2370487248 hasConceptScore W2370487248C179926584 @default.
- W2370487248 hasConceptScore W2370487248C185592680 @default.
- W2370487248 hasConceptScore W2370487248C202416437 @default.
- W2370487248 hasConceptScore W2370487248C41895202 @default.
- W2370487248 hasConceptScore W2370487248C54355233 @default.
- W2370487248 hasConceptScore W2370487248C55493867 @default.
- W2370487248 hasConceptScore W2370487248C555283112 @default.
- W2370487248 hasConceptScore W2370487248C67705224 @default.
- W2370487248 hasConceptScore W2370487248C81885089 @default.
- W2370487248 hasConceptScore W2370487248C86803240 @default.
- W2370487248 hasConceptScore W2370487248C95911042 @default.
- W2370487248 hasIssue "1" @default.
- W2370487248 hasLocation W23704872481 @default.
- W2370487248 hasOpenAccess W2370487248 @default.
- W2370487248 hasPrimaryLocation W23704872481 @default.
- W2370487248 hasRelatedWork W1501820245 @default.
- W2370487248 hasRelatedWork W1849644714 @default.
- W2370487248 hasRelatedWork W1927058072 @default.
- W2370487248 hasRelatedWork W1972790981 @default.
- W2370487248 hasRelatedWork W1985704210 @default.
- W2370487248 hasRelatedWork W1997115294 @default.
- W2370487248 hasRelatedWork W2037064308 @default.
- W2370487248 hasRelatedWork W2129223696 @default.
- W2370487248 hasRelatedWork W2163301533 @default.
- W2370487248 hasRelatedWork W2388714568 @default.
- W2370487248 hasVolume "21" @default.
- W2370487248 isParatext "false" @default.
- W2370487248 isRetracted "false" @default.
- W2370487248 magId "2370487248" @default.
- W2370487248 workType "article" @default.