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- W2370498518 abstract "Objective:Preparing specificity chicken egg yolk antibody(IgY) against enterovirus 71(EV71) and detecting its biological activities,this is significant for prevention and treatment of EV71 infection.Methods:Four hens were immunized three times with the purified human EV71 virus as an antigen.The immunoglobulin Y(IgY) antibody was extracted and purified from the egg yolk by water combine polyethylene glycol(water-peg) method.The yield and molecular mass of IgY were analysed by Bradford method and SDS-PAGE electrophoresis.ELISA and Agar double immunodiffusion were used for detect the titer of IgY,while the immunoreactivity of anti-EV71 IgY was tested using Western blot.Results:The yield and purity of IgY were 7.76 mg/ml and 94.86% respectively.Results of ELISA indicated that the specific anti-EV71 IgY antibody appeared in the egg yolk 10 days after primary immunization and reached the highest titer 1∶20 480 after 40 days,while the titer of Agar double immunodiffusion was 1∶16.Western blot analysis revealed that the purified IgY antibody owned good immunoreactivity and a specifically combination capacity of EV71 virus.Conclusion:Egg yolk is a large scale source of antidodys specifically against EV71 virus.The anti-EV71 IgY extracted and purified by Water-PEG method possess good production,purity,and biological activities." @default.
- W2370498518 created "2016-06-24" @default.
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- W2370498518 date "2011-01-01" @default.
- W2370498518 modified "2023-09-25" @default.
- W2370498518 title "Preparation and identification of specific chicken egg yolk immunoglobulins against enterovirus 71" @default.
- W2370498518 hasPublicationYear "2011" @default.
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