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- W2370564868 abstract "Objective:To establish a quantitative method for determining the contents of four nucleosides in Banlangen Keli by UPLC.Method:The column was Agilent Zorbax SB C18(4.6 mm×50 mm,1.8 μm),the mobile phase consisted of water(A) and methanol(B) with linear gradient elution(0-4 min,0-3%B;4-12 min,3-12%B;12-16 min,50-50%B;16-20 min,0-0%B),the flow rate was 0.6 mL·min-1,the column temperature was 30 ℃,Detection wavelength was 254 nm.Result:The linear range of uridine,guanosine,epigoitrin and adenosine were 2.164-86.56 μg (r=0.9997);2.49-99.6 μg(r=0.9999);3.696-147.84 μg(r=0.9999);2.476-99.040 μg(r=0.9999),.Their average recoveries were 95.9%,99.9%,101.0%,99.8% with RSD=0.8%,1.8%,1.3%,0.8%%,respectively(n=6)。.The contents of nucleosides were different in samples of five productive enterprises.Conclusion:The method is efficient,good reproducible,sensitive,and accurate." @default.
- W2370564868 created "2016-06-24" @default.
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- W2370564868 date "2010-01-01" @default.
- W2370564868 modified "2023-09-28" @default.
- W2370564868 title "UPLC determination of the main nucleosides in Banlangen granules" @default.
- W2370564868 hasPublicationYear "2010" @default.
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