Matches in SemOpenAlex for { <https://semopenalex.org/work/W2370570851> ?p ?o ?g. }
Showing items 1 to 64 of
64
with 100 items per page.
- W2370570851 endingPage "7" @default.
- W2370570851 startingPage "1394" @default.
- W2370570851 abstract "To explore the effects of Huqi Extractum (HQE) on the viability and apoptosis in mouse thymic lymphocytes against 60Co radiation.Thymic lymphocytes were isolated from 4 -8 weeks healthy male Kunming mice and primarily cultured. Then they were divided into the control group, the irradiation group, the low dose HQE group, the medium dose HQE group, and the high dose HQE group. Equal volume of serum free RPMI-1640 culture solution was added in the control group and the irradiation group, while equal volume of HQE solution (at the daily dose of 25, 50, and 100 mg/mL) was respectively added in the low, medium, and high dose HQE groups. Except the control group, those in the rest groups were exposed radiation at a single dose of 5 Gy gamma-ray. Changes of the thymic lymphocytes' viability were measured by MTT colorimetric assay at 12, 24, 36, and 48 h after radiation. The early apoptosis rate was detected using flow cytometry (FCM) after 10-h radiation. The apoptosis was detected using agarose gel electrophoresis to observe the DNA injury after 24-h radiation.The viability level decreased more obviously in the irradiation group than in the control group at 24 -48 h after radiation (P < 0.01, P < 0.05). The average viability level was obviously higher in the low, medium, and high dose HQE groups than in the irradiation group (P < 0.05) in a dose dependent manner. The early apoptosis rate was obviously lower in the low, medium, and high dose HQE groups than in the irradiation group, with statistical difference shown in the high dose HQE group (P < 0.01). Typical DNA ladder fragments were found in the electrophoresis in all groups except the control group. But the DNA injury was comparatively milder in the low, medium, and high dose HQE groups, with more obvious effects shown in the high dose HQE group.HQE showed protection for the viability of early thymic lymphocytes exposed to the 60CO radiation, and could lower the early apoptosis level." @default.
- W2370570851 created "2016-06-24" @default.
- W2370570851 creator A5031505569 @default.
- W2370570851 creator A5052377229 @default.
- W2370570851 creator A5077289752 @default.
- W2370570851 date "2012-10-01" @default.
- W2370570851 modified "2023-09-23" @default.
- W2370570851 title "[Effects of huqi extractum on the viability and apoptosis in mouse thymic lymphocytes against 60Co radiation]." @default.
- W2370570851 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23163154" @default.
- W2370570851 hasPublicationYear "2012" @default.
- W2370570851 type Work @default.
- W2370570851 sameAs 2370570851 @default.
- W2370570851 citedByCount "0" @default.
- W2370570851 crossrefType "journal-article" @default.
- W2370570851 hasAuthorship W2370570851A5031505569 @default.
- W2370570851 hasAuthorship W2370570851A5052377229 @default.
- W2370570851 hasAuthorship W2370570851A5077289752 @default.
- W2370570851 hasConcept C111337013 @default.
- W2370570851 hasConcept C121332964 @default.
- W2370570851 hasConcept C153911025 @default.
- W2370570851 hasConcept C16685009 @default.
- W2370570851 hasConcept C185544564 @default.
- W2370570851 hasConcept C190283241 @default.
- W2370570851 hasConcept C2778271344 @default.
- W2370570851 hasConcept C2989005 @default.
- W2370570851 hasConcept C552990157 @default.
- W2370570851 hasConcept C553184892 @default.
- W2370570851 hasConcept C55493867 @default.
- W2370570851 hasConcept C71924100 @default.
- W2370570851 hasConcept C86803240 @default.
- W2370570851 hasConceptScore W2370570851C111337013 @default.
- W2370570851 hasConceptScore W2370570851C121332964 @default.
- W2370570851 hasConceptScore W2370570851C153911025 @default.
- W2370570851 hasConceptScore W2370570851C16685009 @default.
- W2370570851 hasConceptScore W2370570851C185544564 @default.
- W2370570851 hasConceptScore W2370570851C190283241 @default.
- W2370570851 hasConceptScore W2370570851C2778271344 @default.
- W2370570851 hasConceptScore W2370570851C2989005 @default.
- W2370570851 hasConceptScore W2370570851C552990157 @default.
- W2370570851 hasConceptScore W2370570851C553184892 @default.
- W2370570851 hasConceptScore W2370570851C55493867 @default.
- W2370570851 hasConceptScore W2370570851C71924100 @default.
- W2370570851 hasConceptScore W2370570851C86803240 @default.
- W2370570851 hasIssue "10" @default.
- W2370570851 hasLocation W23705708511 @default.
- W2370570851 hasOpenAccess W2370570851 @default.
- W2370570851 hasPrimaryLocation W23705708511 @default.
- W2370570851 hasRelatedWork W1511425935 @default.
- W2370570851 hasRelatedWork W1536173831 @default.
- W2370570851 hasRelatedWork W2357267659 @default.
- W2370570851 hasRelatedWork W2363034077 @default.
- W2370570851 hasRelatedWork W2374397084 @default.
- W2370570851 hasRelatedWork W2374966914 @default.
- W2370570851 hasRelatedWork W2380700939 @default.
- W2370570851 hasRelatedWork W2392170322 @default.
- W2370570851 hasRelatedWork W2394354805 @default.
- W2370570851 hasRelatedWork W3029184733 @default.
- W2370570851 hasVolume "32" @default.
- W2370570851 isParatext "false" @default.
- W2370570851 isRetracted "false" @default.
- W2370570851 magId "2370570851" @default.
- W2370570851 workType "article" @default.