Matches in SemOpenAlex for { <https://semopenalex.org/work/W2370606119> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W2370606119 abstract "The C-terminal (comprising 105 amino acids) of neutral amino acid transporter ASCT2 (named as TAIL) is the largest extracellular domain of the protein.ASCT2 is reported to be the main receptor for the envelope of human endogenous retrovirus W family (syncytin) on cell membrane.ASCT2 cDNA was derived from human breast cancer cell line MCF-7 by RT-PCR method.DNA fragment encoding the TAIL peptide was inserted into pET-41b vector and the recombinant plasmid was expressed in Escherichia coli(E.coli).TAIL peptide,which was recombinated with a glutathione S-transferase (GST) at the N-terminal and a His6 tag at the C-terminal,was expressed in E.coli cells.SDS-PAGE and Western blotting analysis demonstrated that the recombinant protein existed both in the supernatant and the pellet part of E.coli lysates.The supernatant was further purified by affinity chromatography.Highly pure recombinant protein could bind with MCF-7 cells which were reported to express syncytin molecules on their membranes.These results suggest that the recombinant protein has the potential to bind with syncytin molecules." @default.
- W2370606119 created "2016-06-24" @default.
- W2370606119 creator A5028926069 @default.
- W2370606119 date "2010-01-01" @default.
- W2370606119 modified "2023-09-23" @default.
- W2370606119 title "Expression,Purification and Identification of Human Recombinant ASCT2 C-Terminal Extracellular Domain in Escherichia coli" @default.
- W2370606119 hasPublicationYear "2010" @default.
- W2370606119 type Work @default.
- W2370606119 sameAs 2370606119 @default.
- W2370606119 citedByCount "0" @default.
- W2370606119 crossrefType "journal-article" @default.
- W2370606119 hasAuthorship W2370606119A5028926069 @default.
- W2370606119 hasConcept C104317684 @default.
- W2370606119 hasConcept C10858879 @default.
- W2370606119 hasConcept C123894998 @default.
- W2370606119 hasConcept C153911025 @default.
- W2370606119 hasConcept C179247698 @default.
- W2370606119 hasConcept C181199279 @default.
- W2370606119 hasConcept C187882448 @default.
- W2370606119 hasConcept C2779281246 @default.
- W2370606119 hasConcept C28406088 @default.
- W2370606119 hasConcept C33161422 @default.
- W2370606119 hasConcept C40767141 @default.
- W2370606119 hasConcept C547475151 @default.
- W2370606119 hasConcept C55493867 @default.
- W2370606119 hasConcept C86803240 @default.
- W2370606119 hasConceptScore W2370606119C104317684 @default.
- W2370606119 hasConceptScore W2370606119C10858879 @default.
- W2370606119 hasConceptScore W2370606119C123894998 @default.
- W2370606119 hasConceptScore W2370606119C153911025 @default.
- W2370606119 hasConceptScore W2370606119C179247698 @default.
- W2370606119 hasConceptScore W2370606119C181199279 @default.
- W2370606119 hasConceptScore W2370606119C187882448 @default.
- W2370606119 hasConceptScore W2370606119C2779281246 @default.
- W2370606119 hasConceptScore W2370606119C28406088 @default.
- W2370606119 hasConceptScore W2370606119C33161422 @default.
- W2370606119 hasConceptScore W2370606119C40767141 @default.
- W2370606119 hasConceptScore W2370606119C547475151 @default.
- W2370606119 hasConceptScore W2370606119C55493867 @default.
- W2370606119 hasConceptScore W2370606119C86803240 @default.
- W2370606119 hasLocation W23706061191 @default.
- W2370606119 hasOpenAccess W2370606119 @default.
- W2370606119 hasPrimaryLocation W23706061191 @default.
- W2370606119 hasRelatedWork W1979478786 @default.
- W2370606119 hasRelatedWork W1982165732 @default.
- W2370606119 hasRelatedWork W2041808233 @default.
- W2370606119 hasRelatedWork W2063932294 @default.
- W2370606119 hasRelatedWork W2076183276 @default.
- W2370606119 hasRelatedWork W2079410422 @default.
- W2370606119 hasRelatedWork W2080761623 @default.
- W2370606119 hasRelatedWork W2091044983 @default.
- W2370606119 hasRelatedWork W2144930259 @default.
- W2370606119 hasRelatedWork W2260749553 @default.
- W2370606119 hasRelatedWork W2349423150 @default.
- W2370606119 hasRelatedWork W2354736340 @default.
- W2370606119 hasRelatedWork W2356623277 @default.
- W2370606119 hasRelatedWork W2371423532 @default.
- W2370606119 hasRelatedWork W2371751578 @default.
- W2370606119 hasRelatedWork W2378093612 @default.
- W2370606119 hasRelatedWork W2380771211 @default.
- W2370606119 hasRelatedWork W2394333668 @default.
- W2370606119 hasRelatedWork W2407480472 @default.
- W2370606119 hasRelatedWork W2886600742 @default.
- W2370606119 isParatext "false" @default.
- W2370606119 isRetracted "false" @default.
- W2370606119 magId "2370606119" @default.
- W2370606119 workType "article" @default.