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- W2370638725 abstract "To produce human epidermal growth factor (hEGF) in transgenic mini-tomatoes, plasmids p2300-2A11-hEGF carrying the hEGF gene were constructed and transformed via eletro-perforation to Agrobacterium C58, and then transfected to tomatoes cg2001-14 via leaf disk transformation. The regenerated plantlets were obtained from the cotyledons after shoot and root inducing cultivations. The PCR analysis showed that the hEGF gene has been integrated into the mini-tomato genome. Radioimmunoassay (RIA) showed that the mean yield of rhEGF in the fresh fruits of the transgenic mini-tomatoes amounted to 3.85 ± 0.86 ng /g fruit. Ingestion via gastric intubation of the transgenic mini-tomato juice significantly protected the mice from the alcohol-induced acute peptic ulceration. The ulcer index, expressed as a degree of the stomach lesion, decreased from 52.20±23.24 to 21.20±6.84,and the inhibition rate got to 59.39%." @default.
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- W2370638725 date "2006-01-01" @default.
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- W2370638725 title "Studies of rhEGF Transgenic Mini-tomato" @default.
- W2370638725 hasPublicationYear "2006" @default.
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