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- W2370672018 abstract "Objective:To establish optimized high performance liquid chromatographic method for determination of the drug loading and in vitro release of norvancomycin-bonded Dacron prostheses.Methods:In vitro release of norvancomycin from norvancomycin-bonded Dacron prostheses was achieved by the prostheses being soaked and shaken in phosphate saline buffer. The chromatographic separation was achieved on a Luna C18 column using triethylamine buffer pH3.2- acetonitrile-tetrahydrofuran [92∶7∶1 (v∶v∶v)] as mobile phase. Samples were eluted gradiently with the mobile phase at flow rate 1.0 ml/min and detected at 254nm.Results:The linear range was 10～80μg/ml with correlation coefficients 0.99999. The average recovery was 99.3%(RSD=0.56%). The quantification limit was 0.1ng.Conclusion:The optimized HPLC method is spetific and accurate with low quantification limit." @default.
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- W2370672018 date "2005-01-01" @default.
- W2370672018 modified "2023-09-24" @default.
- W2370672018 title "An optimized HPLC method for determination of the drug loading and release of norvancomycin-bonded Dacron prostheses in vitro" @default.
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