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- W2370698045 abstract "To develop engineered vaccine of deer Brucella,two pairs of primers were designed to amplify the gene according to the pub-lished Brucella ribosomal L7/L12 and OMP31 gene.PCR products were ligated into pGEM-T easy vector,then transformed into compe-tent DH5αfor sequencing and analyzing these genes.Two target genes,L7/L12 and OMP31,were cloned from the Brucella vaccine strain.L7/L12 had 97.1%,and OMP31 had 100% according to the homology analysis.Based on the immunogenicity analysis we knowthat it can be used as a immune advantage antigen." @default.
- W2370698045 created "2016-06-24" @default.
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- W2370698045 date "2009-01-01" @default.
- W2370698045 modified "2023-09-25" @default.
- W2370698045 title "Cloning,Sequencing,Immunogenicity Analyzing of Brucella gene L7/L12 And OMP31" @default.
- W2370698045 hasPublicationYear "2009" @default.
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