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- W2370715545 abstract "A simple and practical method was optimized to overcome the limitations of current method by which HMW DNA was prepared from leaf powder.Root tips of synchronized meristem were homogenized to make preparing suspension containing intact metaphase chromosomes and nuclei,which were further isolated by sucrose gradient centrifugation and flow sorting,respectively.Finally,HMW DNA was prepared with deproteinization,digestion and dialysis of nuclei and chromosomes.The results showed that concentration of the HMW DNA from 200 root tips(10 plugs)was about 4-20 ngμL(4 ngμL in chromosomes DNA and 20 ngμL in nuclei DNA).The average insert fragment size was more than 100 kb after linage,transformation and insert fragment examination by pulse field gel electrophoresis for HMW DNA from nuclei.The similar results were obtained by HMW DNA prepared from chromosomes.It is confirmed that the method is applicable to prepare HMW DNA for construction of BAC library." @default.
- W2370715545 created "2016-06-24" @default.
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- W2370715545 date "2006-01-01" @default.
- W2370715545 modified "2023-09-25" @default.
- W2370715545 title "High Molecular Weight DNA Preparation from Nuclei or Chromosomes of Root Meristem of Wheat" @default.
- W2370715545 hasPublicationYear "2006" @default.
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