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- W2370731782 abstract "A pair of primers for the VP7gene of bluetongue virus(BTV)was designed based on the sequences from Genbank.RT-PCR was used to amplify the VP7fragment of BTV,and then the fragment was subcloned to a prokaryotic expression vector pPROEXHTb to construct the targeting vector pPROEXHTb-VP7.Targeting vector was transformed to BL21competent bacteria,and IPTG was added to induce the expression of VP7protein.The protein was identified after purification SDS-PAGE and Westernblotting.The results showed that the molecular weight of VP7was 35ku,and the purified protein could react specifically to VP7monoclonal antibodies." @default.
- W2370731782 created "2016-06-24" @default.
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- W2370731782 date "2013-01-01" @default.
- W2370731782 modified "2023-09-23" @default.
- W2370731782 title "Prokaryotic expression and reactivity analysis of bluetongue virus VP7gene" @default.
- W2370731782 hasPublicationYear "2013" @default.
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