Matches in SemOpenAlex for { <https://semopenalex.org/work/W2370753221> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W2370753221 abstract "[Objective]To construct a single chain variable fragment(scFv)directed against chloramphenicol(scFvCAP).[Methods]The scFvCAP gene was assembled by overlap PCR and optimized by synonymous mutation,then cloned into pET21a and pET32a which is a fusion expression vector with Trx gene at the terminal 5' of foreign gene for expression in Escherichia coli.The inclusion protein of scFvCAP was refolded by β-cyclodextrin companion system.[Results]The scFvCAP gene(Genebank ID:GU258048)was assembled.Under the control of strong promoter T7,the scFvCAP gene was failed to be individually expressed,but over-expressed when fused to Trx gene.An optimized scFvCAP mut(Genebank ID:GU258048)was cloned by reducing the content of G and C from 60.0% to 36.7% in the terminal 5' of 30 base pairs,and over-expressed was as inclusions.The inclusion protein of scFvCAP was successfully refolded byβ-cyclodextrin companion system with an antibody titer similar to its parent monoclonal antibody(MAb).[Conclusion]We successfully construct a over-production system of scFvCAP." @default.
- W2370753221 created "2016-06-24" @default.
- W2370753221 creator A5034583906 @default.
- W2370753221 date "2011-01-01" @default.
- W2370753221 modified "2023-09-24" @default.
- W2370753221 title "OVER-PRODUCTION OF SINGLE CHAIN VARIABLE FRAGMENT DIRECTED AGAINST CHLORAMPHENICOL" @default.
- W2370753221 hasPublicationYear "2011" @default.
- W2370753221 type Work @default.
- W2370753221 sameAs 2370753221 @default.
- W2370753221 citedByCount "0" @default.
- W2370753221 crossrefType "journal-article" @default.
- W2370753221 hasAuthorship W2370753221A5034583906 @default.
- W2370753221 hasConcept C104317684 @default.
- W2370753221 hasConcept C111829193 @default.
- W2370753221 hasConcept C123894998 @default.
- W2370753221 hasConcept C153911025 @default.
- W2370753221 hasConcept C159654299 @default.
- W2370753221 hasConcept C185592680 @default.
- W2370753221 hasConcept C203750385 @default.
- W2370753221 hasConcept C2776967038 @default.
- W2370753221 hasConcept C2778473333 @default.
- W2370753221 hasConcept C33161422 @default.
- W2370753221 hasConcept C40767141 @default.
- W2370753221 hasConcept C501593827 @default.
- W2370753221 hasConcept C542903549 @default.
- W2370753221 hasConcept C54355233 @default.
- W2370753221 hasConcept C547475151 @default.
- W2370753221 hasConcept C86803240 @default.
- W2370753221 hasConceptScore W2370753221C104317684 @default.
- W2370753221 hasConceptScore W2370753221C111829193 @default.
- W2370753221 hasConceptScore W2370753221C123894998 @default.
- W2370753221 hasConceptScore W2370753221C153911025 @default.
- W2370753221 hasConceptScore W2370753221C159654299 @default.
- W2370753221 hasConceptScore W2370753221C185592680 @default.
- W2370753221 hasConceptScore W2370753221C203750385 @default.
- W2370753221 hasConceptScore W2370753221C2776967038 @default.
- W2370753221 hasConceptScore W2370753221C2778473333 @default.
- W2370753221 hasConceptScore W2370753221C33161422 @default.
- W2370753221 hasConceptScore W2370753221C40767141 @default.
- W2370753221 hasConceptScore W2370753221C501593827 @default.
- W2370753221 hasConceptScore W2370753221C542903549 @default.
- W2370753221 hasConceptScore W2370753221C54355233 @default.
- W2370753221 hasConceptScore W2370753221C547475151 @default.
- W2370753221 hasConceptScore W2370753221C86803240 @default.
- W2370753221 hasLocation W23707532211 @default.
- W2370753221 hasOpenAccess W2370753221 @default.
- W2370753221 hasPrimaryLocation W23707532211 @default.
- W2370753221 hasRelatedWork W2114431190 @default.
- W2370753221 hasRelatedWork W2352105286 @default.
- W2370753221 hasRelatedWork W2352896374 @default.
- W2370753221 hasRelatedWork W2357751149 @default.
- W2370753221 hasRelatedWork W2368672550 @default.
- W2370753221 hasRelatedWork W2370397273 @default.
- W2370753221 hasRelatedWork W2374697450 @default.
- W2370753221 hasRelatedWork W2375997749 @default.
- W2370753221 hasRelatedWork W2378383639 @default.
- W2370753221 hasRelatedWork W2380228977 @default.
- W2370753221 hasRelatedWork W2386935393 @default.
- W2370753221 hasRelatedWork W2391523632 @default.
- W2370753221 hasRelatedWork W2392175294 @default.
- W2370753221 hasRelatedWork W2392317406 @default.
- W2370753221 hasRelatedWork W2411552976 @default.
- W2370753221 hasRelatedWork W2462812236 @default.
- W2370753221 hasRelatedWork W2985211432 @default.
- W2370753221 hasRelatedWork W3146252746 @default.
- W2370753221 hasRelatedWork W3150311667 @default.
- W2370753221 hasRelatedWork W87092271 @default.
- W2370753221 isParatext "false" @default.
- W2370753221 isRetracted "false" @default.
- W2370753221 magId "2370753221" @default.
- W2370753221 workType "article" @default.