Matches in SemOpenAlex for { <https://semopenalex.org/work/W2370784656> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2370784656 abstract "OBJECTIVE To observe the inhibitory effect of Targeted inhibition of Bcl-2 gene expression by Hsa-miR-15a/16-1 on the growth of nasopharyngeal carcinoma CNE-2 cells.METHODS The recombinant plasmid pENTR-CMV-EGFP-hsa-mir-15a/16-1 which had been successfully constructed was transfected into CNE-2 cells with the help of Lipofectamine 2000.The expression of miR-15a/16-1,Bcl-2 mRNA was detected by RT-qPCR.The levels of Bcl-2 and Caspase-3 Protein were measured by Western blot and FCM.The inhibitory effect of Bcl-2 gene on growth of CNE-2 cells was detected by CCK-8.Apoptosis of the CNE-2 cells was observed by analyzing of nucleus morphology with DAPI pigmentation.RESULTS After transfection of the recombinant plasmid,the expression of both miR15a and miR-16-1 increased significantly(F=547.525,P 0.001;F =99.979,P 0.001),the expression of Bcl-2 mRNA had no significant difference(F=0.220,P 0.05),the expression of Bcl-2 protein decreased,the expression of activated Caspase-3 protein increased.Apoptosis of CNE-2 cell in vitro was obvious.Meanwhile the cell proliferation was inhibited after transfection of the recombinant plasmid,and the effects showed an obvious time-effect relationship.CONCLUSION Targeted inhibition of Bcl-2 gene expression by Hsa-miR-15a/16-1 could inhibit cells proliferation and promote apoptosis on human nasopharyngeal carcinoma CNE-2 cells in vitro." @default.
- W2370784656 created "2016-06-24" @default.
- W2370784656 creator A5035040618 @default.
- W2370784656 date "2011-01-01" @default.
- W2370784656 modified "2023-09-26" @default.
- W2370784656 title "Inhibitory effect of nasopharyngeal carcinoma CNE-2 cells through targeted inhibitory effect on the Bcl-2 gene expression by Hsa-mir-15a/16-1" @default.
- W2370784656 hasPublicationYear "2011" @default.
- W2370784656 type Work @default.
- W2370784656 sameAs 2370784656 @default.
- W2370784656 citedByCount "0" @default.
- W2370784656 crossrefType "journal-article" @default.
- W2370784656 hasAuthorship W2370784656A5035040618 @default.
- W2370784656 hasConcept C104317684 @default.
- W2370784656 hasConcept C126322002 @default.
- W2370784656 hasConcept C150194340 @default.
- W2370784656 hasConcept C153911025 @default.
- W2370784656 hasConcept C185592680 @default.
- W2370784656 hasConcept C190283241 @default.
- W2370784656 hasConcept C2776415932 @default.
- W2370784656 hasConcept C2777527632 @default.
- W2370784656 hasConcept C2778997737 @default.
- W2370784656 hasConcept C2779707156 @default.
- W2370784656 hasConcept C40767141 @default.
- W2370784656 hasConcept C49923114 @default.
- W2370784656 hasConcept C509974204 @default.
- W2370784656 hasConcept C54009773 @default.
- W2370784656 hasConcept C55493867 @default.
- W2370784656 hasConcept C62112901 @default.
- W2370784656 hasConcept C71924100 @default.
- W2370784656 hasConcept C86803240 @default.
- W2370784656 hasConcept C92087593 @default.
- W2370784656 hasConceptScore W2370784656C104317684 @default.
- W2370784656 hasConceptScore W2370784656C126322002 @default.
- W2370784656 hasConceptScore W2370784656C150194340 @default.
- W2370784656 hasConceptScore W2370784656C153911025 @default.
- W2370784656 hasConceptScore W2370784656C185592680 @default.
- W2370784656 hasConceptScore W2370784656C190283241 @default.
- W2370784656 hasConceptScore W2370784656C2776415932 @default.
- W2370784656 hasConceptScore W2370784656C2777527632 @default.
- W2370784656 hasConceptScore W2370784656C2778997737 @default.
- W2370784656 hasConceptScore W2370784656C2779707156 @default.
- W2370784656 hasConceptScore W2370784656C40767141 @default.
- W2370784656 hasConceptScore W2370784656C49923114 @default.
- W2370784656 hasConceptScore W2370784656C509974204 @default.
- W2370784656 hasConceptScore W2370784656C54009773 @default.
- W2370784656 hasConceptScore W2370784656C55493867 @default.
- W2370784656 hasConceptScore W2370784656C62112901 @default.
- W2370784656 hasConceptScore W2370784656C71924100 @default.
- W2370784656 hasConceptScore W2370784656C86803240 @default.
- W2370784656 hasConceptScore W2370784656C92087593 @default.
- W2370784656 hasLocation W23707846561 @default.
- W2370784656 hasOpenAccess W2370784656 @default.
- W2370784656 hasPrimaryLocation W23707846561 @default.
- W2370784656 hasRelatedWork W2000192089 @default.
- W2370784656 hasRelatedWork W2174721279 @default.
- W2370784656 hasRelatedWork W2305822846 @default.
- W2370784656 hasRelatedWork W2351826146 @default.
- W2370784656 hasRelatedWork W2357089570 @default.
- W2370784656 hasRelatedWork W2400320840 @default.
- W2370784656 hasRelatedWork W2405815479 @default.
- W2370784656 hasRelatedWork W2414484538 @default.
- W2370784656 hasRelatedWork W2414813853 @default.
- W2370784656 hasRelatedWork W2468615007 @default.
- W2370784656 hasRelatedWork W2472620452 @default.
- W2370784656 hasRelatedWork W2495333804 @default.
- W2370784656 hasRelatedWork W2517172560 @default.
- W2370784656 hasRelatedWork W2578718647 @default.
- W2370784656 hasRelatedWork W2981322774 @default.
- W2370784656 hasRelatedWork W3024753914 @default.
- W2370784656 hasRelatedWork W3031256895 @default.
- W2370784656 hasRelatedWork W3032752645 @default.
- W2370784656 hasRelatedWork W3144198455 @default.
- W2370784656 hasRelatedWork W3207130751 @default.
- W2370784656 isParatext "false" @default.
- W2370784656 isRetracted "false" @default.
- W2370784656 magId "2370784656" @default.
- W2370784656 workType "article" @default.