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- W2370790809 abstract "The anti-nematode(Alcaligenes faecalis)strain BC2000 was chromosomally tagged with the marker genes gfp and luxAB by electroporation with a pUTmini-Tn5 tmnsposon vector containing the gfp/luxAB dual markers.Transformants were screened for the green fluorescence phenotype of the gfp marker by fluorescence stereomicroscopy and one tagged strain was selected for further experiments and designated as strain BC2001.A 700 bp fragment of the gfp gene was amplified from the BC2001 genome DNA using two gfp specific primers.Morphologically the cells of the engineered sWain were similar to the original sWain as determined by laser scanning confocal microscopy.Luciferase activity of BC2001 increased rapidly during log-phase growth in LB medium,but decreased in stationary phase.These results confirmed that the dual marker was insetted successfully into the BC2000 genome through electroporation,and highly expressed in transformant BC2001 under the control ofpsbA promoter." @default.
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- W2370790809 date "2004-01-01" @default.
- W2370790809 modified "2023-09-22" @default.
- W2370790809 title "Transformation and Expression Detection of gfp/luxAB Dual-marker System on Anti-nematode(Alcaligenes faecalis) Strain BC2000" @default.
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