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- W2370795891 abstract "Aim To obtain the short peptides through screening phage random peptide library with sera from schistosomiasis patients. Methods Specific Ig was purified from sera of patients with chronic schistosomiasis and used to immunoscreen a phage random peptide library of 7 anmino acid residues displayed as a fusion protein on shell protein III of filamentous phage M13. The positive clones were obtained through three rounds of biopanning; the reactivity of each clone bound to specific Ig was examined by ELISA. The specific clones were used to examine sera from schistosomiasis patients by Dot ELISA. Results The eluted phages were enriched nearly to 100 fold through three rounds of biopanning. In 30 phage clones selected randonly from the third round biopanning, 26 clones of them could bind to the specific Ig. Two clones with highest A595 value in positive clones had a potential role in immunodiagnosis of schistosomiasis. Conclusion Schistosome specific antigenic epitopes can be identified by the technique of immunoscreening phage random peptide library using infected patient sera and these epitopes pay the foundation for preparing immunodiagnostic reagent or vaccine." @default.
- W2370795891 created "2016-06-24" @default.
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- W2370795891 date "2000-01-01" @default.
- W2370795891 modified "2023-09-24" @default.
- W2370795891 title "Immunoscreening of phage random peptide library with sera from patients with schistosomiasis japonica" @default.
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